Aac/Aap CRISPR/Cas12b(C2c1)
Carbomenu has protein expression platform in both prokaryotic cells (E.coil) and eukaryotic cells (Yeast/CHO-k1/293). Carbomenu is able to deal with kilogram level customized protein production orders. Our product line can be optimized according to customer orders to meet personalized needs.
Information
Known as: | C2c1 |
Species： | Aas/Aap |
Cat.No.: | GY4002 |
Nuclear localization signal: | With NLS or without NLS |
Expression Host： | Ecoli. |
Package: | 500pmol、1000pmol、5000pmol |
Price: | |
500pmol | Inquiry |
1000pmol | Inquiry |
5000pmol | Inquiry |
Introduction
Cas12b nuclease (also known as C2c1) is an RNA-mediated endonuclease, target double stranded DNA can be cleaved specifically in the presence of target DNA PAM. Cas12b protein is smaller than Cas9 and Cas12a. Aac Cas12b is derived from Alicyclobacillus acidoterrestris. Aap Cas12b Derived from Alicyclobacillus Acidophilus acidophilic. Aap Cas12b was more suitable for the one-tube system combined with LAMP. Cas12b has the best cutting reaction temperature of 48 ℃. Similar with Cas12a from the same family, Cas12b recognizes PAM sequences of 5'-TTN and cleaves DNA to produce sticky ends. However Cas12b is double RNA Guidance, dependent on crRNA and tracrRNA (or sgRNA formed by linking). In addition, Cas12b can be applied to more than just targets DsDNA cutting. Cas12b can also be used for rapid detection of target nucleic acid.
Product Component
Package | PKG1 | PKG2 |
Cas12b Nuclease | 500pmol | 1000pmol |
10×Cas12b Buffer | 1mL×5 | 1mL×10 |
Control Target DNA and crRNA | 2 rxn×5 | 2 rxn×10 |
Recommended reaction system（20ul）
Component | Volume |
10×Cas12b Buffer | 2μL |
2.5μM crRNA | 2μL |
1μM Cas12b Nuclease | 1μL |
30 nM substrate DNA | 2μL |
Nuclease-free water up to | 20μL |
Incubate at 48℃ for 0.5-2 h,65℃ 5min | |
FOR RESEARCH USE ONLY
References
1. Defining the seed sequence of the Cas12b CRISPR-Cas effector complex. Jain I, Minakhin L, Mekler V, Sitnik V, Rubanova N, Severinov K, Semenova E.RNA Biol. 2019 Apr;16(4):413-422.
2. The application of a heat-inducible CRISPR/Cas12b (C2c1) genome editing system in tetraploid cotton (G. hirsutum) plants. Wang Q, Alariqi M, Wang F, Li B, Ding X, Rui H, Li Y, Xu Z, Qin L, Sun L, Li J, Zou J, Lindsey K, Zhang X, Jin S.Plant Biotechnol J. 2020 Dec;18(12):2436-2443.
