Recombinant human IL/ Interleukin
Interleukins (IL) are a type of cytokine first thought to be expressed by leukocytes alone but have later been found to be produced by many other body cells. They play essential roles in the activation and differentiation of immune cells, as well as proliferation, maturation, migration, and adhesion. They also have pro-inflammatory and anti-inflammatory properties. The primary function of interleukins is, therefore, to modulate growth, differentiation, and activation during inflammatory and immune responses. Interleukins consist of a large group of proteins that can elicit many reactions in cells and tissues by binding to high-affinity receptors in cell surfaces. They have both paracrine and autocrine function. Interleukins are also used in animal studies to investigate aspect related to clinical medicine.
Information
IL | Expression Host | Construction | Tag |
IL1A | HEK293 cells | Ser113-Ala271 | His tag / hFc tag |
IL2 | Ala21-Thr153 | His tag / hFc tag | |
IL3 | Ala20-Phe152 | His tag / hFc tag | |
IL4 | His25-Ser153 | His tag / hFc tag | |
IL6 | Val30-Met212 | His tag / hFc tag | |
IL7 | Asp26-His177 | His tag / hFc tag | |
IL10 | Ser19-Asn178 | His tag / hFc tag | |
IL12 P70-P35 | Arg23-Ser219 | His tag | |
IL12 P70-P40 | Ile23-Ser328 | His tag | |
IL12 P80-P40 | Ile23-Ser328 | His tag | |
IL15 | Asn49-Ser162 | His tag / hFc tag | |
IL18 | Tyr37-Asp193 | His tag / hFc tag | |
IL21 | His25-Ser162 | His tag / hFc tag |
Package
1g,10g,100g,1kg, just support package above 1g.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS，5%mannital，0.01% Tween80 pH7.4.
Quality Control
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Storage
Lyophilized protein should be stored and transported at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
FOR RESEARCH USE ONLY
References
1.The Role of Interleukin 6 During Viral Infections. auro Velazquez-Salinas, Antonio Verdugo-Rodriguez, Luis L. Rodriguez1 and Manuel V. Borca1.Front. Microbiol., 10 May 2019.
2.Serum levels of interleukin-6 are linked to the severity of the disease caused by Andes Virus. Angulo, J., Martinez-Valdebenito, C., Marco, C., Galeno, H., Villagra, E., Vera, L., et al. (2017). PLoS Negl. Trop. Dis. 11:e0005757.
2.Interleukins (from IL-1 to IL-38), interferons, transforming growth factor β, and TNF-α: Receptors, functions, and roles in diseases.Mübeccel Akdis, Alar Aab, Can Altunbulakli, Oliver F. Wirz,Josefina Sierra Zakzuk,Cezmi A. Akdis.REVIEWS AND FEATURE ARTICLE| VOLUME 138, ISSUE 4, P984-1010.
3.Evolutionary divergence and functions of the human interleukin (IL) gene family. Brocker C, Thompson D, Matsumoto A, Nebert DW, Vasiliou V.Human Genomics.Oct 2010 5 (1): 30–55.
4.Astrocytes support hippocampal-dependent memory and long-term potentiation via interleukin-1 signaling. Ben Menachem-Zidon O, Avital A, Ben-Menahem Y, Goshen I, Kreisel T, Shmueli EM, Segal M, Ben Hur T, Yirmiya R. Brain, Behavior, and Immunity. Jul 2011,25 (5): 1008–16.
