Recombinant human C5a
C5a encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the C5 alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mutations in this gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]
Information
Known as: | Complement C5 a chain,C5a anaphylatoxin,C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 |
Source： | Homo sapiens (Human) |
Cat.No.: | CM7004 |
Construction： | Leu679-Arg751 |
Tag: | His tag (C-terminus) |
Expression Host： | HEK293 cells |
Formulation
Lyophilized from 0.22 μm filtered solution in PBS，5%mannital，0.01% Tween80 pH7.4.
Quality Control
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Storage
Lyophilized protein should be stored and transported at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
FOR RESEARCH USE ONLY
